Data Entry

During the course of the assay, experimental data is entered by laboratory staff directly into the Cell Counts and Plate Data window which is conveniently separated into three tabbed pages.

The first page is used to record cell counts and this data is used by the program to automatically compute the next dilution required. Alternatively, the cell counts can be set to use assumed default values. Osmolality and pH values can also be entered here as well as free text comments and postfix codes to indicate precipitation etc. The Survival Plates tab allows entry of wells lost and number of negative wells per plate. Cell Count Factor, Relative Survival and Cloning Efficiency are computed and displayed.

The second page has a single tab for the user to record the cell counts that have now been reached, and again assists the user with sub-culture by computing the next dilution required. Additional observations/comments can be added for each culture flask as appropriate.

The third page has three tabs for Cell counts, Viability Plates and Mutant Selection Plates. The final recorded cell counts are entered and the program calculates Total Suspension Growth and Relative Suspension Growth. The user can then indicate whether a specific treatment or control is to be plated for viability and mutant selection.

The Viability plates tab allows entry of negative wells and wells lost and calculates Relative Total Growth and Cloning Efficiency. Similarly, the Mutant Selection Plates tab allows entry of large and small colony wells. Size ratio of small/large wells, Mutant Frequency, Cloning Efficiency and fold increase are all computed & displayed. The program also automatically highlights if the Global Evaluation Factor has been exceeded.

During the course of an assay, experiment worksheets in Microsoft Word format can be generated by the program and printed off to assist with the practical aspects of sub-culturing and scoring.

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