Mouse Lymphoma Assay

The L5178Y mouse lymphoma assay, using the thymidine kinase (tk) gene, is the most widely used in vitro mammalian cell gene mutation assay. There are two methods for performing the assay, one using soft agar medium for cloning and enumeration of mutants, and the other using liquid medium and 96-well microwell plates. Perceptive Instruments have supplied colony sizing and data processing systems for the soft agar version of the assay for over a decade. Our current solution for soft agar MLA utilises the colony counting and sizing capabilities of the Sorcerer image analysis system.For the microwell version of the assay, we have the MLA Study Manager.

Study management & reporting software for Microwell MLA

The MLA Study Manager is a management and reporting system for the microwell mouse lymphoma assay. This program is designed to be fully compliant with GLP and FDA 21 CFR Part 11.

It will be possible to to share relevant study, test compound and other information with the Ames Study Manager for centralised data management.

Soft agar mouse lymphoma colony sizing system

The Sorcerer Colony Counting system is an ideal solution for the Soft Agar version of the Mouse Lymphoma Assay. The system uses a purpose built Petri-viewer to obtain a high contrast image of the culture dish. Sorcerer produces a complete size distribution based upon colony area or diameter.

The system has the ability to save data to Microsoft Excel, Microsoft Access and Oracle databases.