Mouse Lymphoma Assay
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The L5178Y mouse lymphoma assay, using the thymidine
kinase (tk) gene, is the most widely used in vitro mammalian cell
gene mutation assay. There are two methods for performing the
assay, one using soft agar medium
for cloning and enumeration of mutants, and the other using liquid
medium and 96-well microwell plates.
Perceptive Instruments have supplied colony
sizing and data processing systems for the soft agar version
of the assay for over a decade. Our current solution for soft
agar MLA utilises the colony counting and sizing capabilities
of the Sorcerer image analysis system.For the microwell version of the assay, we have the MLA Study Manager.
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Study management & reporting software for Microwell
MLA
The MLA Study Manager is a management
and reporting system for the microwell mouse lymphoma assay. This
program is designed to be fully compliant
with GLP and FDA 21 CFR Part 11.
It will be possible to to share relevant study, test compound and
other information with the Ames Study Manager for centralised
data management. |
Soft agar mouse lymphoma colony sizing system
The Sorcerer Colony Counting system
is an ideal solution for the Soft Agar version of the Mouse Lymphoma
Assay. The system uses a purpose built Petri-viewer to obtain
a high contrast image of the culture dish. Sorcerer produces a
complete size distribution based
upon colony area or diameter.
The system has the ability to save data to Microsoft Excel, Microsoft
Access and Oracle databases.
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MLA Study Manager
overview | Sorcerer Colony Counter overview
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