Mouse Lymphoma Assay

The L5178Y mouse lymphoma assay, using the thymidine kinase (tk) gene, is the most widely used in vitro mammalian cell gene mutation assay. There are two methods for performing the assay, one using soft agar medium for cloning and enumeration of mutants, and the other using liquid medium and 96-well microwell plates. Perceptive Instruments have supplied colony sizing and data processing systems for the soft agar version of the assay for over a decade. Our current solution for soft agar MLA utilises the colony counting and sizing capabilities of the Sorcerer image analysis system.

Sorcerer Colony Counter

Soft agar mouse lymphoma colony sizing system

The Sorcerer Colony Counting system is the ideal solution for the Soft Agar version of the Mouse Lymphoma Assay. The system uses a purpose built Petri-viewer to obtain a high contrast image of the culture dish. Sorcerer produces a complete size distribution based upon colony area or diameter.

The system has the ability to save data to Microsoft Excel and Oracle databases.

More information about Sorcerer Colony Counter

Sorcerer Colony Counter for MLA

• Colony sizing by area or diameter and classification into up to 50 classes
• Instant accurate automatic plate counting for microbial and mammalian cultures.
• Additional applications - Ames, UDS, V79, HPRT, SBA, OPKA.
• Sophisticated image processing with advanced colony separation techniques
• Meet regulatory requirements with Sorcerer’s comprehensive compliance options
• Oracle database connectivity for optimal security of result and audit data
• Powerful Excel macros for analysis and comparison of data
• Oracle database connectivity for optimal security of result and audit data
• GLP and FDA 21 CFR Part 11 compliance with audit trails, user permissions and database security
• Comprehensively tested and validated in accordance with our Quality Management System which is approved to ISO 9001:2008 incorporating ISO 90003

Sorcerer Colony Counter overview